Two movies showing GFP expression in dividing cells in the AB (left) and D (right) founder cell lineages in embryos of strain UL129, generated by transformation with a pes-1::gfp fusion.
Expression is first seen after 100 minutes of development when there are 44 cells in the embryo. Therefore the first few seconds of the movies appear blank until expression begins. Ian Hope captured these images in monochrome, but false colour has been added to the movie concentrating on the AB lineage, to mimic the colour of GFP. (See Hope, I.A. Development 120, 505 – 514 (1994).)
A movie showing early embryonic development (recorded in brightfield by Andrew Lynch).
The QTVR movie of a UL129 embryo (below) was captured by Bill Mohler in his laboratory in Connecticut. (For more examples of Bill’s images visit his cytoshow web site). The left video rotates around the anterior-posterior axis, while the right video rotates around the dorsal-ventral axis, as embryonic development proceeds. Nuclear-localized GFP expression is seen in the AB lineage and then the D lineage and finally Z1 and Z4, with cell divisions in each lineage. (See Hope, I.A. Development 120, 505 – 514 (1994).)